Applications from Indian nationals are invited for temporary engagement under the
following project. Engagement shall be purely on a contractual basis. The details of the Plant Molecular Biology Job are given below. Apply online.
Title of the Project – Designing bidirectional promoter capable of driving cas9 gene and
guide RNA simultaneously for generating CRISPR- Cas9 based knockouts in soybean.
Post – Junior Research Fellow
Funding Agency – SERB
Duration of the Tenure – Two years from the date of service and further on satisfactory
performance
No. of Post – One
Emoluments Fixed per month (Rs.) – Rs. 31000/- + 24% HRA for first two years
Rs.35000/+24% HRA for third year based on satisfactory performance
Essential Qualifications – Post graduate degree in Plant Molecular biology/Biotechnology /Biochemistry/Bioinformatics/Life Sciences/Plant physiology selected through a process
described in any one of the following.
- Scholars who are selected through National Eligibility Test CSIR, UGC-NET including lectureship (Assistant professorship) and GATE.
- The selection process through national level exam conducted by Central government departments and their agencies and institute such as DST, DBT, DAE, DOS, DRDO, MHRD, ICAR, IIT, IISc, IISER etc.
Desirable Qualifications – Hands on working experience in plant tissue culture and
molecular in plant tissue
Date of Interview – 11/09/2023 at 11:00 am
Terms & Conditions:
- Interested candidates will attend the Walk-In-Interviews with 2 copies of Application
form along with 2 – attested copies of all the documents related to educational qualifications/experience/publications etc. The candidates must also bring all the
Original documents for verification. However, the candidates who wish to take
online interviews must submit their Application form strictly in the prescribed format along with the scanned pdf of their original documents by dated 05/09/2023. The application form and the documents must be clubbed in a single pdf and send it on the email IDs viz. [email protected], [email protected] and [email protected]. The subject of the e-mail should be kept as the Advertisement No. of the post. - The link for taking the interviews will be sent on the email ID of the eligible
candidates only. - The Original of following documents, along with 2 self-attested copies, mentioned
below must be brought by the candidate to secure their eligibility for taking
Interviews.
- Application form strictly in the format given in the next page (all fields mandatory)
- Complete CV.
- Class 10 Mark sheet
- Class 10 Pass Certificate
- Class 12 Mark sheet
- Class 12 Pass Certificate
- Graduation Mark sheet/Transcript
- Graduation Degree Certificate
- Post-Graduation Mark sheet/Transcript
- Post-Graduation Degree Certificate
- Experience Certificate (as & where applicable)
- No-Objection Certificate (essentially required if currently employed, as & where applicable)
- Conversion factor from CGPA to percentage, wherever applicable.
- PhD. Degree certificate. (as & where applicable)
- PhD marksheet. (as & where applicable)
- SC/ST/OBC Non-Creamy Layer/PH certificate as applicable.
- Copy of publication if any.
- Any other relevant documents.
4. Age Limit: Upper age limit for application to the post of JRF is mentioned as under (As
per ICAR guidelines)
- Male – 35 years
- Female – 40 years
5. The positions are purely temporary, on a contractual basis and initially offered for
one year or till project duration whichever is earlier. However, the positions can be
terminated with one months’ notice owing to any change in circumstances. The person
selected will have no right to claim employment or engagement in ICAR at the end of the
contract period.
6. The tentative date of interview 11/09/2023. The candidates shall report at LBS
Building, Pusa Campus, New Delhi – 110012 at 9.30 am.
Hello friends, we are providing you some of the interview questions and answers that you can expect for the interview for Plant Molecular Biology Job. Please prepare and do well at your interview Plant Molecular Biology Job.
Question: Can you explain your understanding of bidirectional promoters and their significance in the context of this project?
Answer: Bidirectional promoters are regulatory regions of DNA that initiate the transcription of two adjacent genes in opposite directions. In our project, the bidirectional promoter is crucial as it needs to drive both the Cas9 gene and the guide RNA simultaneously. This design allows for precise control of CRISPR-Cas9-based gene editing in soybean, ensuring that the Cas9 protein and guide RNA are expressed in the same cells at the same time to achieve targeted knockout of specific genes.
Question: How do you plan to select the optimal bidirectional promoter for this project, and what factors will you consider in your selection?
Answer: To select the optimal bidirectional promoter, I would first conduct a comprehensive literature review to identify existing promoters with proven effectiveness in plant systems, particularly soybean. I will assess factors such as promoter strength, specificity to soybean, and minimal off-target effects. Additionally, I’ll consider the ease of cloning and compatibility with the CRISPR-Cas9 system.
Question: Could you describe your experience with CRISPR-Cas9 technology and how you envision its application in generating knockouts in soybean?
Answer: I have a strong background in CRISPR-Cas9 technology, including both its molecular biology aspects and practical applications. In this project, I will design guide RNAs specific to the target genes in soybean and co-express them with the Cas9 gene under the bidirectional promoter. By delivering this CRISPR-Cas9 system into soybean cells, we can induce double-stranded breaks at the target gene loci, which will trigger the plant’s DNA repair mechanisms, often leading to gene knockout mutations.
Question: Can you share your experience with plant tissue culture and molecular biology techniques, and how do you plan to integrate these skills into the project?
Answer: I have extensive hands-on experience in plant tissue culture and molecular biology techniques, which are vital for this project. Plant tissue culture will be employed to maintain and propagate soybean cells and plants in a controlled environment. Molecular biology techniques, such as PCR, gel electrophoresis, and DNA sequencing, will be used to verify successful gene editing events. Additionally, I will utilize my skills to optimize the transformation and regeneration protocols for soybean to achieve efficient knockout results.
Question: How do you plan to ensure that your research aligns with the objectives of the SERB-funded project, and how will you demonstrate satisfactory performance during the tenure?
Answer: To align my research with the project’s objectives, I will establish clear milestones and deliverables in consultation with my supervisor. Regular progress meetings and reporting will ensure that my work remains on track. I will actively seek opportunities for collaboration and knowledge exchange within the research community to enhance project outcomes. My commitment to the project’s success, as well as meeting these milestones and delivering quality results, will demonstrate my satisfactory performance during the two-year tenure and beyond.
