IISER Mohali Hiring Short-Term Project Student – MSc Life Sciences Apply

IISER Mohali Short-Term Project Student – MSc Life Sciences Apply

IISER Mohali Short-Term Project Student – MSc Life Sciences Apply. MSc Life Sciences Junior Research Fellow. IISER Mohali Careers. IISER Mohali Short-term Project JRF. Interested and eligible applicants can check out all of the details on the same below

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Application for a short-term project student / junior research fellow (JRF) in a research project on RNA splicing

Positions: 1

Project Title: Function of a Splicing Factor in Yeast

Outline of the project: The project aims to understand the function of an RNA splicing factor. The project would utilise experimental approaches in molecular biology, genetics and biochemistry in yeast.

Duration: 3 months (extendable up to 6 months)

How to Apply: Candidates should send their application as a composite pdf file with their CV, proof of academic qualification and research experience to Dr Shravan Kumar Mishra ([email protected]). Since the position is for a short duration aimed at completing a project, only candidates with the abovementioned experience would be considered. Shortlisted candidates will be called for online interviews in the third week of August 2023.

Application Deadline: Monday, August 14, 2023.

Emoluments: As per GoI

Essential Qualifications: Master in any branch of Life Sciences with a good academic record and a minimum of one-year research experience working with project(s) involving genetics and biochemistry in yeast. Expertise in protein-protein interaction by co-immunoprecipitation experiments and RNA splicing experiments is required.

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Possible Interview Questions:

1. Can you explain your experience in working with genetics and biochemistry in yeast-based research projects? Provide specific examples of the projects you have worked on and the techniques you utilized. Answer: I have a Master’s degree in Life Sciences, and I have been involved in yeast-based research projects for the past year. In one project, I investigated the genetic interactions between specific genes in yeast strains to understand their role in RNA splicing. In another project, I conducted co-immunoprecipitation experiments to study protein-protein interactions in yeast cells.
2. How familiar are you with RNA splicing experiments? Can you describe the methods you have used for RNA splicing analysis in previous research projects? Answer: Yes, I am quite familiar with RNA splicing experiments. In my previous research, I used RT-PCR and gel electrophoresis techniques to analyze splicing patterns of specific genes in yeast. Additionally, I performed in vitro splicing assays to investigate the impact of various factors on the splicing process.
3. The project requires expertise in protein-protein interaction studies using co-immunoprecipitation. Can you elaborate on your experience in conducting co-immunoprecipitation experiments? Answer: Certainly, I have hands-on experience with co-immunoprecipitation experiments. In one of my projects, I successfully immunoprecipitated the target protein using specific antibodies and then probed for its interacting partners through Western blot analysis. I also optimized the conditions to minimize nonspecific binding and ensure reliable results.
4. As this is a short-term project, time management is crucial. How do you plan to ensure the timely completion of the research objectives within the 3 to 6-month duration? Answer: To ensure timely completion, I would first create a detailed research plan, breaking down the objectives into smaller achievable tasks with specific deadlines. I will regularly assess the progress and adjust the plan if necessary. Effective communication with the team and efficient coordination of experiments will be key to meeting the project’s timeline.
5. The project requires expertise in experimental approaches in molecular biology, genetics, and biochemistry. Can you provide an example of an integrated approach you have used in a previous research project to study a biological process? Answer: Certainly, in a previous project, I investigated the regulatory network of a specific gene involved in RNA splicing in yeast. I combined molecular biology techniques like gene cloning and mutagenesis to generate different gene variants. Then, I employed genetics by creating mutant yeast strains and analyzed their phenotypes. Finally, I used biochemical assays to study the impact of these mutations on protein-protein interactions and splicing efficiency, resulting in a comprehensive understanding of the gene’s function

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