MSc Biochem SRF Job at National Institute of Immunology – Attend Walk-In

NII MSc Biochem SRF Job Opening – Attend Wlak-In-Interview

NII MSc Biochem SRF Job Opening – Attend Wlak-In-Interview. MSc Biochemistry Senior Research Fellow job opening. Interested and eligible applicants can check out all of the details on the same below

Hi, since you are interested in the role of Senior Research Fellow at the National Institute of Immunology, check out all of the possible interview questions that you may have to face and the answers below.

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Name of the Posts – Senior Research Fellow (Project)

No. of Posts – (One position only)

Name of the Project – “Glycoproteomic profiling of O-glycosylation of leukosialin (CD43/sialophorin) by mass spectrometry and its role in immunological processes” funded by SERB

Name of the PI – Dr. Srinivasa-Gopalan Sampathkumar,
Staff Scientist-VI
Chemical Glycobiology Laboratory.

Email ID of Project Investigator: [email protected].

Duration: Six-months (6 months)

How to Apply –

1. CANDIDATE MAY FILL UP THE APPLICATION FORM IN THE PRESCRIBED FORMAT ALONG WITH NECESSARY DOCUMENTS FOR VERIFICATION.
2. APPLICATION FORMS CONTAINING INCOMPLETE INFORMATION SHALL NOT BE ENTERTAINED.
3. THE DATE OF PASSING THE EXAMINATIONS MUST BE INDICATED CLEARLY.
4. ONLY REGISTERED CANDIDATES WILL BE INTERVIEWED.
5. NO TA/DA WILL BE PAID FOR ATTENDING THE INTERVIEW.

• Date of Walk-In-Interview: 02nd August 2023 (Wednesday)
• Registration of the Candidates: 9:00 AM to 10:00 AM
• Time of Interview: 10:00 AM to 1:00 PM

Educational Qualifications – M.Sc in Biochemistry, Organic Chemistry, or Chemistry.

Desirable: Experience of at least three years in mammalian cell cultures, biochemical techniques, mass spectrometry, and knowledge of glycobiology and host-pathogen interactions.

Job Description: To investigate glycosylation of CD antigens in mammalian cells, isolate, and characterize them using the glycoproteomic methodologies. Mammalian cell culture, western / lectin blotting, confocal microscopy, flow cytometry, peptide and glycopeptide synthesis, and bio-analytical techniques.

Emoluments – Rs. 35,000/- per month + 24% HRA (As per DST OM dated 30.01.2019)

Check the notification and the application format below

Hi, since you are interested in the role of Senior Research Fellow at the National Institute of Immunology, check out all of the possible interview questions that you may have to face and the answers below.

1. Question: Can you explain your experience with mammalian cell cultures and how you have applied this knowledge in your previous work? Answer: During my previous research projects, I have been extensively involved in working with mammalian cell cultures. I have hands-on experience in maintaining various cell lines, optimizing growth conditions, and conducting experiments to study cellular processes. I have also utilized cell culture techniques to investigate the impact of glycobiology in host-pathogen interactions, which aligns well with the objectives of this project.
2. Question: How familiar are you with mass spectrometry and its application in glycoproteomic profiling? Could you provide an example of a study where you utilized mass spectrometry for glycoprotein analysis? Answer: I am well-versed with mass spectrometry techniques, particularly in the context of glycoprotein analysis. In one of my previous projects, we employed mass spectrometry to study O-glycosylation patterns of a specific protein, similar to the objectives of this project. We successfully identified glycopeptides and determined their glycosylation sites, which greatly contributed to our understanding of immunological processes.
3. Question: Explain the importance of glycosylation in CD antigens and its relevance to immunological processes. Answer: Glycosylation plays a critical role in modulating the functions of CD antigens, influencing various aspects of immunological processes. It affects the stability, trafficking, and signaling properties of these cell surface proteins. By studying the glycosylation patterns of CD antigens, we can gain insights into their functional significance and their involvement in immune responses.
4. Question: Have you worked with biochemical techniques like western blotting and lectin blotting? How have these methods contributed to your previous research? Answer: Yes, I have utilized biochemical techniques such as western blotting and lectin blotting in my previous research. These methods have been instrumental in characterizing glycoproteins, including CD antigens, by detecting glycan structures. By analyzing lectin binding patterns and western blot profiles, we could identify specific glycosylation modifications and understand their potential roles in cellular processes.
5. Question: Can you explain the significance of peptide and glycopeptide synthesis in the context of this project? How can these techniques aid in glycosylation studies? Answer: Peptide and glycopeptide synthesis are essential for studying glycosylation events and mapping glycosylation sites. By chemically synthesizing specific peptides and glycopeptides, we can create reference standards for mass spectrometry analysis, facilitating the identification and quantification of glycosylation modifications. These synthesized molecules also enable us to investigate the impact of specific glycan structures on protein functions, providing valuable insights into the glycoproteomic profiling of CD antigens.

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