ACTREC MSc Biotech, Life Sciences, Botany & Zoology Project Coordinator Job

Here are five possible interview questions along with their answers for the Senior Project Coordinator position:

1. Question: Can you discuss your experience with DNA extraction and quantification techniques in a research setting? Answer: I have extensive experience in DNA extraction and quantification. I have successfully performed these techniques in various research projects, employing methods such as phenol-chloroform extraction, column-based purification, and automated extraction platforms. I am also skilled in using spectrophotometry and fluorometry for DNA quantification.
2. Question: Have you worked with RT-PCR/PCR? Please elaborate on your familiarity and expertise in these techniques. Answer: Yes, I have considerable experience with RT-PCR and PCR techniques. I have designed and optimized primer sets, prepared reaction mixtures, and conducted PCR amplifications for gene expression studies. I am proficient in setting up thermal cycling conditions, analyzing gel electrophoresis results, and interpreting data generated from these techniques.
3. Question: Can you describe your experience using multichannel pipettes and their role in laboratory work? Answer: I am well-versed in using multichannel pipettes for efficient and precise reagent dispensing. I have experience with 8-12 multichannel pipettes and understand the importance of proper calibration and technique to ensure accurate volume delivery. I have used them extensively in multiwell plate setups for various assays and experiments.
4. Question: Are you familiar with performing ELISA assays? Please elaborate on your experience and knowledge in this area. Answer: Yes, I have practical experience in conducting ELISA assays. I am proficient in both direct and indirect ELISA formats, including plate coating, sample preparation, antibody incubation, and detection methods such as colorimetric or fluorescent readouts. I am also adept at analyzing ELISA data and interpreting results.
5. Question: Have you been involved in GWAS or Next Generation Sequencing experiments? Could you provide an overview of your experience in this field? Answer: Yes, I have worked on research projects involving GWAS and Next Generation Sequencing. I have contributed to experimental design, sample preparation, library preparation, sequencing runs, and initial data analysis. I am familiar with bioinformatics tools used for variant calling, data filtering, and functional annotation.

Remember, these are sample questions, and the actual interview questions may vary. It’s crucial to review the job description thoroughly and align your answers with the specific requirements mentioned. Additionally, prepare examples from your previous experience to showcase your skills and competencies relevant to the position.