C-CAMP BTech & MSc Biological Sciences Assistant Job, Apply Online
C-CAMP BTech & MSc Biological Sciences Assistant Job, Apply Online. MSc & Bech Biological Sciences job opening at C-CAMP. Mouse Genome Engineering Facility Assistant job opening. Interested and eligible applicants can check out all of the job details below
Hey there, if you are interested in this Mouse Genome Engineering Facility Assistant vacancy at C-CAMP then check out these possible interview questions and the answers below
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Position: Mouse Genome Engineering Facility Assistant – One Position
About The Post:
The Bangalore Bio-Cluster Mouse Genome Engineering Facility is a well-equipped state-of-the-art functional facility for the generation of transgenic and gene-edited mouse models. Our commitment is to provide animal model resources to all members of the Bangalore Biocluster, including the National Centre for Biological Sciences (NCBS), the Institute for Stem Cell Biology and Regenerative Medicine (inStem), the Tata Institute for Genetics and Society (TIGS), and the Centre for Cellular and Molecular Platforms (C-CAMP), as well as to researchers nationwide. The main operational domains of the MGEF Facility are: 1) using the latest gene-editing tools and technologies to generate customized new mouse models; 2) mouse sperm and embryo
cryo-banking services; 3) access to the latest mouse-assisted reproductive technologies to maintain and recover complicated mouse lines; 4) training & workshops.We are seeking a highly motivated Facility Assistant (MGEF Facility) to support the Mouse Genome Engineering Facility (MGEF) as a molecular biologist as well as laboratory animals-assisted reproductive technologies.
This is a contractual position and the current tenure will be till 31 March 2024. Further extn will be based on the performance evaluation.
Qualification & Experience:
- BE/B.Tech/M.Pharma/Masters in any biological sciences with prior experience in handling of laboratory mice/rats, knowledge/experience in sperm and embryo handling is desirable. Excellent organization, recordkeeping, and English communication skills are essential.
- Coordinate and participate in the daily operations of the mouse genome engineering facility for managing and executing molecular biology activities related to mice model generation
- Experience in molecular biology techniques such as PCR, DNA/RNA extraction, gel electrophoresis, genotyping
- Perform molecular cloning, and gene-targeted vector construction for mice model generation
- Basic understanding of CRISPR technology, designing strategies for the generation of CRISPR-mediated animal model generation, In house CRISPR guide synthesis and validation
- Experience in rederivation, In Vitro Fertilization, genotyping, Mouse sperm and embryo cryopreservation and breeding services
- Colony management, post-surgical animal monitoring and care
- Maintenance of cryo database on animal Colony management software
- Accurate recording keeping as per Standard Operative Procedures.
- Communicating project status and results with clients.
Pay Scale: Based on experience and qualification.
How to Apply:
- Candidates MUST send an updated CV/resume AND a MANDATORY one-page COVER LETTER to [email protected] describing your background and the reason and interest for applying to this position.
- Please note that candidates applying without a proper cover letter will NOT be considered.
The last date is 03 June 2023.
How to Apply – Interested candidates may please apply by clicking on this link- https://forms.gle/tqDLRgoxhzLXE35W6.
Hey there, if you are interested in this Mouse Genome Engineering Facility Assistant vacancy at C-CAMP then check out these possible interview questions and the answers below
- Can you describe your experience and knowledge in handling laboratory mice and rats? How have you applied this expertise in previous molecular biology activities? Answer: I have extensive experience in handling laboratory mice and rats. In previous roles, I have been responsible for managing and executing molecular biology activities related to mice model generation. I have performed tasks such as genotyping, colony management, post-surgical animal monitoring, and care. Additionally, I have utilized my expertise in molecular biology techniques like PCR, DNA/RNA extraction, and gel electrophoresis to support various research projects involving mice models.
- Have you worked with CRISPR technology for the generation of animal models? Can you describe your experience in designing strategies and synthesizing CRISPR guides? Answer: Yes, I have experience working with CRISPR technology for the generation of animal models. I have been involved in designing strategies for CRISPR-mediated animal model generation, including the synthesis and validation of CRISPR guides in-house. I have used my understanding of CRISPR principles and techniques to efficiently edit genes in mice models, contributing to the development of specific phenotypes and research objectives.
- Can you discuss your experience in mouse sperm and embryo handling? Have you performed techniques like in vitro fertilization (IVF) and cryopreservation? Answer: I have hands-on experience in mouse sperm and embryo handling. I have successfully performed techniques such as in vitro fertilization (IVF) to generate embryos for experimental purposes. I am also familiar with cryopreservation methods for preserving mouse sperm and embryos. These skills have allowed me to contribute to maintaining and recovering complicated mouse lines, as well as provide breeding services to support research projects.
- How do you ensure accurate record-keeping and adherence to standard operating procedures (SOPs) in your work? Can you provide examples of how you have maintained records and communicated project status and results? Answer: Accurate record-keeping and adherence to SOPs are crucial aspects of my work. I maintain detailed records of experimental procedures, protocols, and results using electronic databases or laboratory notebooks. I follow SOPs for each step of the molecular biology activities and ensure that all documentation is up-to-date. Additionally, I regularly communicate project status and results with clients and team members through progress reports, meetings, and presentations.
- Can you describe your experience in molecular cloning and gene-targeted vector construction for mice model generation? What techniques and tools have you utilized in this process? Answer: I have significant experience in molecular cloning and gene-targeted vector construction for mice model generation. I have utilized techniques such as restriction enzyme digestion, ligation, transformation, and plasmid purification for cloning purposes. I am proficient in the use of molecular biology tools like PCR, gel electrophoresis, and DNA sequencing to verify the successful construction of gene-targeted vectors. My expertise in these techniques has facilitated the generation of customized mouse models with precise genetic modifications.
Note: It is recommended to tailor the answers to your own experiences and qualifications when preparing for an interview.
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